ENTIRE COURSE NOTES Molecular Biology 1

Description

All lecture notes for the entire subject. Would not have been able to get a HD without these. TOPICS: - Revision, Elements of Gene Structure, Agarose Gel Electrophoresis - Extraction and Purification of Nucleic Acids → RNA + Chromosomal (genetic) DNA - Extraction and Purification of Nucleic Acids → Plasmid DNA - Restriction Endonucleases, DNA Ligation, Transformation + Electroporation - Molecular Cloning Strategies - Expressing Foreign Proteins in E. coli - DNA Sequencing Bioinformatics + Polymerase Chain Reaction - Polymerase Chain Reaction, Applications + Site-directed Mutagenesis - Microbial Genetics + Regulation and Control, the lac Operon - Southern Hybridisation, Northern Blotting and Hybridisation, RT-PCR, Western Blotting and Hybridisation