H1 BCMB20005 (2022) prac+ lecture notes (89% subject top 5% of cohort)

Description

Notes include contents from lectures weeks 1-12 and practical content across the semester. I received an overall 89% mark and was noticed by the coordinator as cohort 5%. I hope my notes can also help you to get high marks in this subject. This is a great subject to gain lab techniques. Absolutely loved it. My notes covered the contents listed below: - pipetting - isolation of DNA - different types of PCR - southern and western blotting - DNA sequence - recombinant plasma and cloning - cell biology (pH, protein, tissue culturing) - protein assay (buret, Bradford, lowry assay) - protein purification techniques (affinity, ion-exchange and size exchange column, immunoprecipitation) -bacterial expression system (bacteria, yeast and insect expression system)